PA5-21996

antibody from Invitrogen Antibodies

Targeting: HMGCL HL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21996

Provider

Invitrogen Antibodies

Product name

HMGCL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: H1299, mouse liver, rat liver.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HMGCL Polyclonal Antibody detects HMGCL protein by Western blot analysis. A. 50 µg mouse liver lysate/extract.10 % SDS-PAGE. HMGCL Polyclonal Antibody (Product # PA5-21996) dilution: 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HMGCL Polyclonal Antibody (Product # PA5-21996). Sample (30 µg of whole cell lysate). A: H1299. 10% SDS PAGE. HMGCL Polyclonal Antibody (Product # PA5-21996) diluted at 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HMGCL Polyclonal Antibody detects HMGCL protein by Western blot analysis. A. 50 µg mouse liver lysate/extract.10 % SDS-PAGE. HMGCL Polyclonal Antibody (Product # PA5-21996) dilution: 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HMGCL Polyclonal Antibody detects HMGCL protein by Western blot analysis. A. 50 µg rat liver lysate/extract.10 % SDS-PAGE. HMGCL Polyclonal Antibody (Product # PA5-21996) dilution: 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HMGCL was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HMGCL Polyclonal Antibody (Product # PA5-21996) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL (Product # PA5-21996) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.