orb38637

antibody from Biorbyt

Targeting: PIK3CA PI3K

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

orb38637

Provider

Biorbyt

Proper citation

Biorbyt Cat#orb38637, RRID:AB_10995952

Product name

Anti-PK3CA (Y317) antibody

Antibody type

Polyclonal

Description

Peptide affinity purified rabbit polyclonal antibody.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

400 μl

Storage

Store at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Western blot analysis of in mouse heart tissue lysates (35ug/lane)using PK3CA (Y317) antibody (primary antibody dilution at: 1:1000)

Immunohistochemistry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin embedded human hepatocarcinoma tissue using PK3CA (Y317) antibody (primary antibody dilution at: 1:50-100)

Flow cytometry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using PK3CA (Y317) antibody (primary antibody dilution at: 1:10-50)