PA5-22367

antibody from Invitrogen Antibodies

Targeting: HSD17B4 DBP, MFE-2, SDR8C1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22367

Provider

Invitrogen Antibodies

Product name

HSD17B4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, mouse brain.

Concentration

0.66 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HSD17B4 Polyclonal Antibody (Product # PA5-22367). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. HSD17B4 Polyclonal Antibody (Product # PA5-22367) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HSD17B4 Polyclonal Antibody (Product # PA5-22367). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. HSD17B4 Polyclonal Antibody (Product # PA5-22367) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-HSD17B4 Polyclonal Antibody (Product # PA5-22367) and an 80 kDa band corresponding to HSD17B4 was observed in all tissue lysates tested except Mouse Brain. Lower expression of SAMHD1 was observed in Mouse Brain as compared to Mouse Testis and Mouse Heart [10.1016/s0960-0760(99)00066-7]. Tissue lysates (30g lysate) of Mouse Testis (Lane 1), Mouse Heart (Lane 2) and Mouse Brain (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Two uncharacterized bands (*) were observed in certain models.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HSD17B4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HSD17B4 Polyclonal Antibody (Product # PA5-22367) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HSD17B4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HSD17B4 Polyclonal Antibody (Product # PA5-22367) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HSD17B4 (Product # PA5-22367) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HSD17B4 (Product # PA5-22367) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.