Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM08479SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM08479SU-N, RRID:AB_2035776
- Product name
- anti MTBP (667-812)
- Antibody type
- Monoclonal
- Antigen
- Recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PHL-1
- Vial size
- 0.1 ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).
