Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB322-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PDGF R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PDGF R beta or recombinant mouse PDGF R alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 35248
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Structural environment built by AKAP12+ colon mesenchymal cells drives M2 macrophages during inflammation recovery.
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry.
Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions.
In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Metabotropic glutamate receptors are expressed in adult human glial progenitor cells.
Yang JM, Lee HS, Seo JH, Park JH, Gelman IH, Lo EH, Kim KW
Scientific reports 2017 Feb 16;7:42723
Scientific reports 2017 Feb 16;7:42723
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry.
Wu Y, Prager A, Boos S, Resch M, Brizic I, Mach M, Wildner S, Scrivano L, Adler B
PLoS pathogens 2017 Apr;13(4):e1006281
PLoS pathogens 2017 Apr;13(4):e1006281
Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair.
Prignano F, Campolmi P, Bonan P, Ricceri F, Cannarozzo G, Troiano M, Lotti T
Dermatologic therapy 2009 Nov;22 Suppl 1:S8-15
Dermatologic therapy 2009 Nov;22 Suppl 1:S8-15
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Ball SG, Shuttleworth CA, Kielty CM
The Journal of cell biology 2007 May 7;177(3):489-500
The Journal of cell biology 2007 May 7;177(3):489-500
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Li R, Maminishkis A, Wang FE, Miller SS
Investigative ophthalmology & visual science 2007 Dec;48(12):5722-32
Investigative ophthalmology & visual science 2007 Dec;48(12):5722-32
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
Blood 2006 Mar 1;107(5):1896-902
Blood 2006 Mar 1;107(5):1896-902
Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions.
Wilson HC, Scolding NJ, Raine CS
Journal of neuroimmunology 2006 Jul;176(1-2):162-73
Journal of neuroimmunology 2006 Jul;176(1-2):162-73
In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
Kübler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
The Prostate 2005 Jun 1;63(4):385-94
The Prostate 2005 Jun 1;63(4):385-94
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Nagineni CN, Kutty V, Detrick B, Hooks JJ
Journal of cellular physiology 2005 Apr;203(1):35-43
Journal of cellular physiology 2005 Apr;203(1):35-43
Metabotropic glutamate receptors are expressed in adult human glial progenitor cells.
Luyt K, Varadi A, Halfpenny CA, Scolding NJ, Molnar E
Biochemical and biophysical research communications 2004 Jun 18;319(1):120-9
Biochemical and biophysical research communications 2004 Jun 18;319(1):120-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PDGF R alpha in Human Breast Cancer Tissue. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PDGF R alpha Monoclonal Antibody (Catalog # MAB322) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by PDGF-AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF R alpha Monoclonal Antibody (Catalog # MAB322). The ND50 is typically 2-10 µg/mL.