AF2114

antibody from R&D Systems

Targeting: PDGFRA CD140a, GAS9, PDGFR2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF2114

Provider

R&D Systems

Product name

Human Phospho-PDGF R alpha (Y742) Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PDGF R alpha when phosphorylated at Y742.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis.

Cavallin LE, Ma Q, Naipauer J, Gupta S, Kurian M, Locatelli P, Romanelli P, Nadji M, Goldschmidt-Clermont PJ, Mesri EA
PLoS pathogens 2018 Jul;14(7):e1007175

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Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry.

Wu Y, Prager A, Boos S, Resch M, Brizic I, Mach M, Wildner S, Scrivano L, Adler B
PLoS pathogens 2017 Apr;13(4):e1006281

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Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.

Ball SG, Bayley C, Shuttleworth CA, Kielty CM
The Biochemical journal 2010 Mar 15;427(1):29-40

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Phospho-PDGF R alpha (Y742) by Western Blot. Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-PDGF R alpha (Y742) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2114), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R alpha (Y742) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.