Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF21141 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-PDGF R alpha (Y762) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PDGF R alpha when phosphorylated at Y762.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Therapeutic potential of TAS-115 via c-MET and PDGFRα signal inhibition for synovial sarcoma.
Yamada S, Imura Y, Nakai T, Nakai S, Yasuda N, Kaneko K, Outani H, Takenaka S, Hamada K, Myoui A, Araki N, Ueda T, Itoh K, Yoshikawa H, Naka N
BMC cancer 2017 May 16;17(1):334
BMC cancer 2017 May 16;17(1):334
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Phospho-PDGF R alpha (Y762) by Western Blot. Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA) or Recombinant Human PDGF-BB (Catalog # 220-BB) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-PDGF R alpha (Y762) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21141), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R alpha (Y762) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.