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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- 44-490G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-c-Kit (Tyr568, Tyr570) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Increased ERK and JNK activities correlate with disease activity in patients with systemic lupus erythematosus.
Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.
Molad Y, Amit-Vasina M, Bloch O, Yona E, Rapoport MJ
Annals of the rheumatic diseases 2010 Jan;69(1):175-80
Annals of the rheumatic diseases 2010 Jan;69(1):175-80
Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.
Shivakrupa R, Linnekin D
Cellular signalling 2005 Jan;17(1):103-9
Cellular signalling 2005 Jan;17(1):103-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition: Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were probed using a Phospho-c-Kit (Tyr568/Tyr570) antibody (Product # 44-490G), followed by incubation with an anti-Rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C-Kit (pY568/pY570) was performed by loading 20 µg of A549 (lane1) and Serum Starved A549 (lane2) cell lysate using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. C-Kit (pY568/pY570) was detected at ~ 170 kDa using C-Kit (pY568/pY570) Rabbit Polyclonal Antibody (Product # 44-490G) at 1:1000 in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD117 (c-Kit) [pYpY568/570] was done on A549 cells treated with EGF (200ng/mL, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with CD117 (c-Kit) [pYpY568/570] Rabbit Polyclonal Antibody (44490G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
