PA5-97454

antibody from Invitrogen Antibodies

Targeting: ALDH7A1 ATQ1, EPD, PDE

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97454

Provider

Invitrogen Antibodies

Product name

ALDH7A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

2.93 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of precipitated ALDH7A1 from Hela whole cell lysate using a ALDH7A1 polyclonal antibody (Product # PA5-97454). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (20µg).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ALDH7A1 using a ALDH7A1 Polyclonal antibody (Product # PA5-97454) at a concentration of 4.7 µg/mL. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 59 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ALDH7A1 in Hela cells using a ALDH7A1 polyclonal antibody (Product # PA5-97454) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ALDH7A1 in Hela cells using a ALDH7A1 polyclonal antibody (Product # PA5-97454) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH7A1 in paraffin embedded human kidney tissue using a ALDH7A1 polyclonal antibody (Product # PA5-97454) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH7A1 in paraffin embedded human liver tissue using a ALDH7A1 polyclonal antibody (Product # PA5-97454) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.