H00051701-M02

antibody from Abnova Corporation

Targeting: NLK

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051701-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051701-M02, RRID:AB_913728

Product name

NLK monoclonal antibody (M02), clone 2B11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant NLK.

Antigen sequence

DEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPK
FDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLC
INPQSAAFKSFISSTVAQPSEMPPSPLVWE


Isotype

IgG

Antibody clone number

2B11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NLK expression in transfected 293T cell line by NLK monoclonal antibody (M02), clone 2B11.Lane 1: NLK transfected lysate (Predicted MW: 57.1 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NLK monoclonal antibody (M02), clone 2B11. Western Blot analysis of NLK expression in human liver.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MAP3K7 and NLK. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-NLK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)