NBP2-22405

antibody from Novus Biologicals

Targeting: GRIN2A GluN2A, NMDAR2A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-22405

Provider

Novus Biologicals

Product name

Mouse Monoclonal NMDAR2A Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 170kDa. Does not react with NR2B.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405] - analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of 170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 170 kDa. Other Band(s): 100 kDa.