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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-14974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAXX Monoclonal Antibody (K.180.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- K.180.3
- Vial size
- 100 µL
- Concentration
- 55 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), MDA-MB-231 (Lane 3), Jurkat (Lane 4), Raji (Lane 5) and Ramos (Lane 6). The blot was probed with anti-DAXX Rabbit Monoclonal Antibody (Product # MA5-14974, 1:500 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to DAXX was detected across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Daxx in extracts from K562 and A20 cell lines using Daxx monoclonal antibody (Product # MA5-14974).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Daxx in HeLa cells using a Daxx monoclonal antibody (Product # MA5-14974) (green). Actin filaments are labeled with a fluorescent red phalloidin.
