MA5-23878

antibody from Invitrogen Antibodies

Targeting: BMP7 OP-1

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23878

Provider

Invitrogen Antibodies

Product name

BMP-7 Monoclonal Antibody (164313)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In ELISAs, this antibody shows approximately 25-50% cross-reactivity with recombinant human (rh) BMP-6 and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8.

Antibody clone number

164313

Concentration

0.5 mg/mL

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BMP-7 in immersion fixed paraffin-embedded sections of human prostate. Samples were incubated in BMP-7 monoclonal antibody (Product # MA5-23878) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BMP-7 in immersion fixed paraffin-embedded sections of human prostate cancer tissue. Samples were incubated in BMP-7 monoclonal antibody (Product # MA5-23878) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of BMP-7 in ATDC5 mouse chondrogenic cell line. Samples were incubated in BMP-7 monoclonal antibody (Product # MA5-23878). Recombinant Human BMP-7 induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP-7 Monoclonal Antibody. The ND50 is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL).