Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-35986 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPAG5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPAG5 Polyclonal Antibody detects SPAG5 protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg A431 whole cell extract. C. 30 µg HeLa whole cell extract. D. 30 µg HepG2 whole cell extract.5 % SDS-PAGE. SPAG5 Polyclonal Antibody (Product # PA5-35986) dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPAG5 Polyclonal Antibody detects SPAG5 protein by western blot analysis. A. 30 µg NT2D1 whole cell extract.5 % SDS-PAGE. SPAG5 Polyclonal Antibody (Product # PA5-35986) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPAG5 Polyclonal Antibody detects SPAG5 protein at spindle by immunofluorescent analysis. Sample: HeLa cells were fixed in 100% MeOH for 5 min. Green: SPAG5 protein stained by SPAG5 Polyclonal Antibody (Product # PA5-35986) diluted at 1:500. Blue: Hoechst 33342 staining.