MAB72441

antibody from Novus Biologicals

Targeting: PKM OIP3, PK3, PKM2, THBP1

Antibody data

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Validation data

Product number: MAB72441 - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal PKM2 Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat PKM-2 in Western blot
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 ug
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

PKM protein structure - MAB72441 shown in red.

Enhanced validation

Submitted by: Novus Biologicals (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PKM2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat PKM2 Monoclonal Antibody (Catalog # MAB72441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of Human, Mouse, and Rat PKM2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human PKM2 Monoclonal Antibody (Catalog # MAB72441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of Human PKM2 by Simple Western^TM. Simple Western lane view shows lysate of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PKM2 at approximately 61 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat PKM2 Monoclonal Antibody (Catalog # MAB72441) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.