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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-82487 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82487, RRID:AB_11012699
- Product name
- Rabbit Polyclonal PKM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PKM2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Toponostics of invasive ductal breast carcinoma: combination of spatial protein expression imaging and quantitative proteome signature analysis.
The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
Toponostics of invasive ductal breast carcinoma: combination of spatial protein expression imaging and quantitative proteome signature analysis.
Röwer C, Ziems B, Radtke A, Schmitt O, Reimer T, Koy C, Thiesen HJ, Gerber B, Glocker MO
International journal of clinical and experimental pathology 2011 Mar 31;4(5):454-67
International journal of clinical and experimental pathology 2011 Mar 31;4(5):454-67
The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas.
Bolander A, Agnarsdóttir M, Strömberg S, Ponten F, Hesselius P, Uhlen M, Bergqvist M
Cancer genomics & proteomics 2008 Nov-Dec;5(6):293-300
Cancer genomics & proteomics 2008 Nov-Dec;5(6):293-300
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKM2 Antibody [NBP1-82487] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
