358505

antibody from BioLegend

Targeting: BCL6 BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

358505

Provider

BioLegend

Proper citation

BioLegend Cat#358505, RRID:AB_2562471

Product name

APC anti-human/mouse Bcl-6

Antibody type

Monoclonal

Antigen

Sequence 226-398 of murine BLC6 fused with GST

Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to T_FH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Red dye

Antibody clone number

7D1

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

13-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Brilliant Violet 421™ and IgD PerCP/Cy5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown was gated on B220-positive cells.

Conjugate

Red dye