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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 648302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#648302, RRID:AB_2063446
- Product name
- Purified anti-mouse/human Bcl-6
- Antibody type
- Monoclonal
- Antigen
- Plasmid vector containing cDNA of the Bcl-6 gene
- Description
- B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family. It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4 °C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Raji (lane 1) and A20 (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Bcl -6 monoclonal antibody, IG191E/A8. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescent system.
- Conjugate
- Unconjugated
