Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-11493 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL6 Monoclonal Antibody (BL6.02 (PG-B6p))
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-11493 targets BCL-6 in IHC (P) and Western blot applications and shows reactivity with Bovine, Human, Ovine, Porcine, Rabbit, Mouse and Rat samples.
- Antibody clone number
- BL6.02 (PG-B6p)
- Concentration
- Conc. Not Determined
Submitted references αvβ6 Integrin Promotes Castrate-Resistant Prostate Cancer through JNK1-Mediated Activation of Androgen Receptor.
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.
E2A-positive gastric MALT lymphoma has weaker plasmacytoid infiltrates and stronger expression of the memory B-cell-associated miR-223: possible correlation with stage and treatment response.
B-cell lymphofollicular infiltrates in mycosis fungoides.
B-cell lymphofollicular infiltrates in mycosis fungoides.
Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity.
Roles of syndecan-1, bcl6 and p53 in diagnosis and prognostication of immunoproliferative small intestinal disease.
Enhancement of clonogenicity of human multiple myeloma by dendritic cells.
Frequent occurrence of BCL6 rearrangements in nodular lymphocyte predominance Hodgkin lymphoma but not in classical Hodgkin lymphoma.
Epstein-Barr virus-infected B cells expanding in germinal centers of infectious mononucleosis patients do not participate in the germinal center reaction.
Lu H, Wang T, Li J, Fedele C, Liu Q, Zhang J, Jiang Z, Jain D, Iozzo RV, Violette SM, Weinreb PH, Davis RJ, Gioeli D, FitzGerald TJ, Altieri DC, Languino LR
Cancer research 2016 Sep 1;76(17):5163-74
Cancer research 2016 Sep 1;76(17):5163-74
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
Akay OM, Aras BD, Isiksoy S, Toprak C, Mutlu FS, Artan S, Oner U, Gulbas Z
Cancer genetics 2014 Mar;207(3):87-93
Cancer genetics 2014 Mar;207(3):87-93
The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.
Demirkesen C, Tüzüner N, Esen T, Lebe B, Ozkal S
Leukemia research 2011 Sep;35(9):1269-72
Leukemia research 2011 Sep;35(9):1269-72
E2A-positive gastric MALT lymphoma has weaker plasmacytoid infiltrates and stronger expression of the memory B-cell-associated miR-223: possible correlation with stage and treatment response.
Liu TY, Chen SU, Kuo SH, Cheng AL, Lin CW
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2010 Nov;23(11):1507-17
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2010 Nov;23(11):1507-17
B-cell lymphofollicular infiltrates in mycosis fungoides.
Ferrara G, Chiarelli C, Simonetti S
Tumori 2010 May-Jun;96(3):487-91
Tumori 2010 May-Jun;96(3):487-91
B-cell lymphofollicular infiltrates in mycosis fungoides.
Ferrara G, Chiarelli C, Simonetti S
Tumori 2010 May-Jun;96(3):487-91
Tumori 2010 May-Jun;96(3):487-91
Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity.
Smit LA, Hallaert DY, Spijker R, de Goeij B, Jaspers A, Kater AP, van Oers MH, van Noesel CJ, Eldering E
Blood 2007 Feb 15;109(4):1660-8
Blood 2007 Feb 15;109(4):1660-8
Roles of syndecan-1, bcl6 and p53 in diagnosis and prognostication of immunoproliferative small intestinal disease.
Vaiphei K, Kumari N, Sinha SK, Dutta U, Nagi B, Joshi K, Singh K
World journal of gastroenterology 2006 Jun 14;12(22):3602-8
World journal of gastroenterology 2006 Jun 14;12(22):3602-8
Enhancement of clonogenicity of human multiple myeloma by dendritic cells.
Kukreja A, Hutchinson A, Dhodapkar K, Mazumder A, Vesole D, Angitapalli R, Jagannath S, Dhodapkar MV
The Journal of experimental medicine 2006 Aug 7;203(8):1859-65
The Journal of experimental medicine 2006 Aug 7;203(8):1859-65
Frequent occurrence of BCL6 rearrangements in nodular lymphocyte predominance Hodgkin lymphoma but not in classical Hodgkin lymphoma.
Wlodarska I, Nooyen P, Maes B, Martin-Subero JI, Siebert R, Pauwels P, De Wolf-Peeters C, Hagemeijer A
Blood 2003 Jan 15;101(2):706-10
Blood 2003 Jan 15;101(2):706-10
Epstein-Barr virus-infected B cells expanding in germinal centers of infectious mononucleosis patients do not participate in the germinal center reaction.
Kurth J, Hansmann ML, Rajewsky K, Küppers R
Proceedings of the National Academy of Sciences of the United States of America 2003 Apr 15;100(8):4730-5
Proceedings of the National Academy of Sciences of the United States of America 2003 Apr 15;100(8):4730-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL-6 was performed by loading 25 µg of Ramos (Lane 1), Daudi (Lane 2) and BAF-3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a BCL-6 monoclonal antibody (Product # MA5-11493) at a dilution of 1:20 (Ramos) and 1:10 (Daudi and BAF-3) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 87-98 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (20 µg lysate) of Ramos (Lane 1), Raji (Lane 2) and U2OS (lane 3). The blots were probed with Anti-BCL-6 Mouse Monoclonal Antibody (Product # MA5-11493, 1:10-1:100 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 79 kDa band corresponding to BCL-6 was observed across cell lines tested expect U2OS. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-6 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes in the germinal center.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of BCL-6 was done on Ramos cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with BCL-6 Mouse Monoclonal Antibody (MA511493, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.