AF-403-NA

antibody from Novus Biologicals

Targeting: IL3 IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-403-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse IL-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-3 is observed.

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Interleukin-3 plays a vital role in hyperoxic acute lung injury in mice via mediating inflammation.

Huang Z, Zhang W, Yang J, Sun F, Zhou H
BMC pulmonary medicine 2018 Oct 30;18(1):164

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Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.

Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4227-34

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Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.

Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4227-34

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A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.

Shih CC, Hu MC, Hu J, Weng Y, Yazaki PJ, Medeiros J, Forman SJ
Blood 2000 Mar 15;95(6):1957-66

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-3 and Neutralization by Mouse IL-3 Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA). The ND₅₀ is typically 0.01-0.03 µg/mL.