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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB711 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine GM-CSF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects porcine GM-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots (reducing conditions), approximately 20% cross-reactivity with recombinant rat GM-CSF is observed and less than 1% cross-reactivity with recombinant human GM-CSF and recombinant mouse GM-CSF is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q29118- Isotype
- IgG
- Antibody clone number
- 111802
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants.
Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Martelet L, Lacouture S, Goyette-Desjardins G, Beauchamp G, Surprenant C, Gottschalk M, Segura M
Pathogens (Basel, Switzerland) 2017 Mar 22;6(1)
Pathogens (Basel, Switzerland) 2017 Mar 22;6(1)
Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
Jeong HJ, Song YJ, Lee SW, Lee JB, Park SY, Song CS, Ha GW, Oh JS, Oh YK, Choi IS
Clinical and vaccine immunology : CVI 2010 Apr;17(4):503-12
Clinical and vaccine immunology : CVI 2010 Apr;17(4):503-12
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Porcine GM-CSF Antibody. Recombinant Porcine GM-CSF (Catalog # 711-PG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM-CSF (40 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Porcine GM-CSF Monoclonal Antibody (Catalog # MAB711). The ND50 is typically 1-4 µg/mL.
