IC615P

antibody from R&D Systems

Targeting: CSF2 GM-CSF, GMCSF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC615P

Provider

R&D Systems

Product name

Human GM-CSF PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human GM-CSF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) GM-CSF, rrGM-CSF, rhM-CSF, rhG-CSF, rhIL-5-R alpha , or rhIL-3 R alpha .

Reactivity

Human

Host

Mouse

Antigen sequence

P04141


Isotype

IgG

Antibody clone number

6804

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.

Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17

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Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.

Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17

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High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis.

Uchida K, Nakata K, Trapnell BC, Terakawa T, Hamano E, Mikami A, Matsushita I, Seymour JF, Oh-Eda M, Ishige I, Eishi Y, Kitamura T, Yamada Y, Hanaoka K, Keicho N
Blood 2004 Feb 1;103(3):1089-98

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of GM-CSF in Human Dendritic Cells by Flow Cytometry. Human dendritic cells treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human TGF-beta 1 (Catalog # 240-B) were stained with Mouse Anti-Human GM-CSF PE-conjugated Monoclonal Antibody (Catalog # IC615P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.