PA1-29162

antibody from Invitrogen Antibodies

Targeting: CDK7 CAK, CAK1, CDKN7, MO15, STK1

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-29162

Provider

Invitrogen Antibodies

Product name

Anti-CDK7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-29163 detects Cullin 3 from mouse, human samples. PA1-29163 has been successfully used in ICC, IF and IP applications. The PA1-29163 immunogen is: Synthetic peptide corresponding to the N-terminus of human Cullin-3 protein.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

1 mg/ml

Storage

4° C, do not freeze

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of Cdk7 was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a Cdk7 polyclonal antibody (Product # PA1-29162). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.