LS-C291076

antibody from LSBio

Targeting: B3GNT3 B3GN-T3, B3GNT-3, beta3Gn-T3, HP10328, TMEM3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C291076

Provider

LSBio

Product name

B3GNT3 / TMEM3 Antibody (aa50-100) LS-C291076

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse

Host

Rabbit

Storage

Store at 2°C to 8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human and Mouse B3GNT3 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-B3GNT3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human B3GNT3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B3GNT3 antibody used for IP at 6 ug per reaction. B3GNT3 was also immunoprecipitated by rabbit anti-B3GNT3 antibody BL16626. For blotting immunoprecipitated B3GNT3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.