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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- AM02057PU-S - Provider product page
- Provider
- OriGene
- Product name
- Bone Sialoprotein (IBSP) (native) mouse monoclonal antibody, clone ID1.2, Purified
- Antibody type
- Monoclonal
- Description
- Bone Sialoprotein (IBSP) (native) mouse monoclonal antibody, clone ID1.2, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IBSP
- Isotype
- IgG
- Antibody clone number
- ID1.2
- Vial size
- 10 µg
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoblot analysis of AM02057PU-N/AM02057PU-S specificty. Native and recombinant Bone proteins were separated by SDS-PAGE and immunoblotted with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S at 1/4000 dilution. This antibody did not detect BSP purified from Porcine Bone, nor Porcine Osteopontin (OPN) or SPARC/Osteonectin (ON). Gogan G et al. (2004) Connect Tissue Res 45(1):60-71
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ELISA standard curve showing measurement of Human BSP in a Sandwich Immunoassay using?Cat.-No AM20240PU-N/AM20240PU-S as a Capture antibody and Cat.-No AM02057PU-N/AM02057PU-S as a Detection antibody. OD was determined at 450 nm. The shown standard curve is for demonstration purposes only and can be influenced by buffer, incubation conditions or dilution of the conjugate.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry image staining of BSP in Paraffin Section of Human Tissues. Sections were incubated with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S (1/2000) and detected using HRP conjugated secondary antibody and ABC Vectastain Elite Kit. DAB was used as the Chromogen. The sections were counterstained with Harris' Hematoxylin. Sections of B: Embryonic Tibia Sections of E: Primary breast Carcinoma Sections of G: Bone metastasis from the same patient were stained with this antibody. Inserted within B and E are Control Sections incubated with Mouse IgG. Scale bar = 20 ?m. Gogan G et al. (2004) Connect Tissue Res 45(1):60-71
- Validation comment
- IHC
