Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- ELISA [1]
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Validation data
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- Product number
- 500829 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#500829, RRID:AB_2562840
- Product name
- Purified anti-human IL-4 (MaxPar® Ready)
- Antibody type
- Monoclonal
- Antigen
- CHO-expressed, recombinant human IL-4
- Description
- IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
- Reactivity
- Human, Porcine
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- MP4-25D2
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human Th2-polarized CD4+ T cells were incubated for 5 hours in media alone (left) or with PMA and Ionomycin (right) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 145Nd anti-CD4 (RPA-T4) and 142Nd anti-IL-4 (MP4-25D2).
- Conjugate
- Unconjugated