MAB304-100

antibody from Novus Biologicals

Targeting: IL4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB304-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL-4.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-4 and Neutralization by Human IL-4 Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304). The ND₅₀ is typically 0.03-0.1 µg/mL.