Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC204F-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-4 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL-4.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P05112.1
- Isotype
- IgG
- Antibody clone number
- 3007
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity.
Functional T-cell responses generated by dendritic cells expressing the early HIV-1 proteins Tat, Rev and Nef.
Alpha1beta1 integrin is crucial for accumulation of epidermal T cells and the development of psoriasis.
Piancone F, Saresella M, Marventano I, La Rosa F, Zoppis M, Agostini S, Longhi R, Caputo D, Mendozzi L, Rovaris M, Clerici M
Scientific reports 2016 Jul 14;6:29699
Scientific reports 2016 Jul 14;6:29699
Functional T-cell responses generated by dendritic cells expressing the early HIV-1 proteins Tat, Rev and Nef.
Allard SD, Pletinckx K, Breckpot K, Heirman C, Bonehill A, Michiels A, van Baalen CA, Gruters RA, Osterhaus AD, Lacor P, Thielemans K, Aerts JL
Vaccine 2008 Jul 4;26(29-30):3735-41
Vaccine 2008 Jul 4;26(29-30):3735-41
Alpha1beta1 integrin is crucial for accumulation of epidermal T cells and the development of psoriasis.
Conrad C, Boyman O, Tonel G, Tun-Kyi A, Laggner U, de Fougerolles A, Kotelianski V, Gardner H, Nestle FO
Nature medicine 2007 Jul;13(7):836-42
Nature medicine 2007 Jul;13(7):836-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-4 in NS0 Mouse Cell Line Transfected with Human IL-4 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human IL-4 was stained with Mouse Anti-Human IL-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC204F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.