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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF584 - Provider product page
- Provider
- R&D Systems
- Product name
- Cotton Rat IL-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects cotton rat IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL-4 is observed, 5% cross-reactivity with recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL-4 and recombinant porcine IL-4 is observed.
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAL18820- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Cotton Rat IL-4 Antibody. Recombinant Cotton Rat IL-4 (Catalog # 584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-4 (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-4 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF584). The ND50 is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL).
