Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-28189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, H1299.
- Concentration
- 0.43 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RFC2 Polyclonal Antibody detects RFC2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 Polyclonal Antibody (Product # PA5-28189) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RFC2 Polyclonal Antibody (Product # PA5-28189). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: Hela. 10% SDS PAGE. RFC2 Polyclonal Antibody . RFC2 Polyclonal Antibody (Product # PA5-28189) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RFC2 Polyclonal Antibody detects RFC2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 Polyclonal Antibody (Product # PA5-28189) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RFC2 in paraformaldehyde-fixed HeLa cells using a RFC2 polyclonal antibody (Product # PA5-28189) at a 1:500 dilution.