Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- 35-8300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Synuclein Monoclonal Antibody (Syn 505)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Syn 505
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Human myeloperoxidase (hMPO) is expressed in neurons in the substantia nigra in Parkinson's disease and in the hMPO-α-synuclein-A53T mouse model, correlating with increased nitration and aggregation of α-synuclein and exacerbation of motor impairment.
Chronic mild stress accelerates the progression of Parkinson's disease in A53T α-synuclein transgenic mice.
Age-dependent α-synuclein aggregation in the Microcebus murinus lemur primate.
Novel antibodies to synuclein show abundant striatal pathology in Lewy body diseases.
Maki RA, Holzer M, Motamedchaboki K, Malle E, Masliah E, Marsche G, Reynolds WF
Free radical biology & medicine 2019 Sep;141:115-140
Free radical biology & medicine 2019 Sep;141:115-140
Chronic mild stress accelerates the progression of Parkinson's disease in A53T α-synuclein transgenic mice.
Wu Q, Yang X, Zhang Y, Zhang L, Feng L
Experimental neurology 2016 Nov;285(Pt A):61-71
Experimental neurology 2016 Nov;285(Pt A):61-71
Age-dependent α-synuclein aggregation in the Microcebus murinus lemur primate.
Canron MH, Perret M, Vital A, Bézard E, Dehay B
Scientific reports 2012;2:910
Scientific reports 2012;2:910
Novel antibodies to synuclein show abundant striatal pathology in Lewy body diseases.
Duda JE, Giasson BI, Mabon ME, Lee VM, Trojanowski JQ
Annals of neurology 2002 Aug;52(2):205-10
Annals of neurology 2002 Aug;52(2):205-10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WT TNM, a recombinant wild-type form of a-Synuclein that has been treated with tetranitromethane, using Ms anti-Nitrated a-Synuclein (Product # 35-8300).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of mouse brain (locus coeruleus) double-labeled with Ms anti-Nitrated a-Synuclein (Product # 35-8300, red) and anti-Tyrosine Hydroxylase (green). Image courtesy of John Trojanowski, MD, PhD, University of Pennsylvania School of Medicine, PA.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Distribution of synuclein and amyloid pathology in mouse lemur brains. (A) Sagittal section of mouse lemur stained with Mayer's hemalum showing midbrain and forebrain regions. Scale bar = 2 mm. (B) Sagittal section of mouse lemur stained with Mayer's hemalun showing distribution of betaA4 (purple stars), alpha-synuclein (pink stars), Phospho-Ser129 alpha-synuclein (orange stars) immunoreactive regions. (C-H) Magnifications of selected alpha-synuclein positive regions in young and old animals boxed in A: cortex (C-D), SNpc (E-F), striatum (G-H). (I-N) Magnifications of selected Phospho-Ser129 alpha-synuclein positive regions in young and old animals boxed in A: cortex (I-J), SNpc (K-L), striatum (M-N). (O-T) Magnifications of selected betaA4 positive regions in young and old animals boxed in A: cortex (O-P), SNpc (Q-R), striatum (S-T); Scale bar = 10 um.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Phosphorylated and nitrated alpha-synuclein immunoreactivity is enhanced in the substantia nigra of old (n = 4) as compared with young (n = 3) mouse lemur. (A) Representative midbrain sections from young and old mouse lemur co-immunostained with tyrosine hydroxylase (TH, red) and an anti-phosphorylated alpha-synuclein (P-Ser129 alpha-synuclein, green). The merged image shows colabeling within a nigral neuron. (B) Double immunofluorescence for nitrated alpha-synuclein (green) in TH-positive neurons (red) in young and old animals. The merged image shows coimmunoreactivity within a nigral neuron. Scale bar for panels A and B = 10 um.