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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-52178 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LEKDEREQLR ILGEKEAKLA QQSQALQELI SELDRRCHSS
- Concentration
- 0.2 mg/mL
Submitted references Sequential ubiquitination of NLRP3 by RNF125 and Cbl-b limits inflammasome activation and endotoxemia.
Tang J, Tu S, Lin G, Guo H, Yan C, Liu Q, Huang L, Tang N, Xiao Y, Pope RM, Rajaram MVS, Amer AO, Ahmer BM, Gunn JS, Wozniak DJ, Tao L, Coppola V, Zhang L, Langdon WY, Torrelles JB, Lipkowitz S, Zhang J
The Journal of experimental medicine 2020 Apr 6;217(4)
The Journal of experimental medicine 2020 Apr 6;217(4)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM21 in human cell line CAPAN-2 using a TRIM21 Polyclonal Antibody (Product # PA5-52178).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM21 in control (vector only transfected HEK293T lysate) and TRIM21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a TRIM21 Polyclonal Antibody (Product # PA5-52178).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TRIM21 in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a TRIM21 Polyclonal Antibody (Product # PA5-52178).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TRIM21 in human cell line HUVEC TERT2 using a TRIM21 Polyclonal Antibody (Product # PA5-52178) shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIM21 in human gallbladder using a TRIM21 Polyclonal Antibody (Product # PA5-52178) shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIM21 in human placenta using TRIM21 Polyclonal Antibody (Product # PA5-52178) shows strong cytoplasmic immunoreactivity in monocytes, as well as moderate positivity in trophoblast.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIM21 in human skeletal muscle using TRIM21 Polyclonal Antibody (Product # PA5-52178) shows only weak positivity in striated muscle fibers as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIM21 in human tonsil using TRIM21 Polyclonal Antibody (Product # PA5-52178) shows moderate to strong cytoplasmic positivity in lymphoid cells.
