Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-7097-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-9 Monoclonal Antibody (MH9A4), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody MH9A4 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4. IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9A4 antibody is not blocked by the MH9D1 antibody, suggesting that the two antibodies bind to unique epitopes. Applications Reported: This MH9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MH9A4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MH9A4
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Transforming growth factor-beta 'reprograms' the differentiation of T helper 2 cells and promotes an interleukin 9-producing subset.
Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children.
Interleukin-9.
Veldhoen M, Uyttenhove C, van Snick J, Helmby H, Westendorf A, Buer J, Martin B, Wilhelm C, Stockinger B
Nature immunology 2008 Dec;9(12):1341-6
Nature immunology 2008 Dec;9(12):1341-6
Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children.
Jenmalm MC, Van Snick J, Cormont F, Salman B
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2001 Oct;31(10):1528-35
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2001 Oct;31(10):1528-35
Interleukin-9.
Renauld JC, Houssiau F, Louahed J, Vink A, Van Snick J, Uyttenhove C
Advances in immunology 1993;54:79-97
Advances in immunology 1993;54:79-97
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Th9-polarized human peripheral blood cells were cultured for 5 hours with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or were stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were then stained intracellularly with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-9 eFluor® 660. Cells were fixed and permeabilized with IC Fixation Buffer (Product # 00-8222-49) and Permeabilization Buffer (10X) (Product # 00-8333-56). Cells in the lymphocyte gate were used for analysis.