Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1350 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha 6/CD49f Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin alpha 2, recombinant mouse (rm) Integrin alpha 3, rhIntegrin alpha 7, and rmIntegrin alpha 7 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P23229- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Beta4 integrin promotes osteosarcoma metastasis and interacts with ezrin.
Wan X, Kim SY, Guenther LM, Mendoza A, Briggs J, Yeung C, Currier D, Zhang H, Mackall C, Li WJ, Tuan RS, Deyrup AT, Khanna C, Helman L
Oncogene 2009 Sep 24;28(38):3401-11
Oncogene 2009 Sep 24;28(38):3401-11
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Integrin alpha 6/CD49f by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin alpha 6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Integrin alpha 6/CD49f by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue (cortex), loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 6/CD49f at approximately 167 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Integrin alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
