Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-52295 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAPGEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SWLEEKEKEV VSALRYFKTI VDKMAIDKKV LEMLPGSASK VLEAILPLVQ NDPRIQHSSA LSSCYSRVYQ SLANLIRWSD QVMLEGVNSE DKEMVTTVKG VIKAVLDGVK ELVRLTIEKQ GRPSPTSPVK PSSPASK
- Concentration
- Lot-Specific
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAPGEF1 in control (vector only transfected HEK293T lysate) and RAPGEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RAPGEF1 Polyclonal Antibody (Product # PA5-52295).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RAPGEF1 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a RAPGEF1 Polyclonal Antibody (Product # PA5-52295).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAPGEF1 in human skeletal muscle shows strong cytoplasmic positivity in myocytes. Samples were probed using a RAPGEF1 Polyclonal Antibody (Product # PA5-52295).
