Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
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Validation data
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- Product number
- F48148 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- KLF4 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 481-504 from the human protein was used as the immunogen for this KLF4 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the KLF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of KLF4 antibody and MDA-MB231, NCI-H460 lysate.Predicted molecular weight: 50-60 kDa + ~75 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:100. Note the highly specific localization of immunoreactivity to the nuclei.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells using KLF4 antibody; Ab was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cells stained with KLF4 antibody at 1:100. Note the highly specific localization of the immunoreactivity to the nuclei but not the cytoplasm.