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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC3640A - Provider product page
- Provider
- R&D Systems
- Product name
- Human KLF4 APC-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse (rm) KLF4 and less than 1% cross-reactivity with recombinant human (rh) KLF5, rhKLF6, and rmKLF15.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Red dye
- Antigen sequence
AAH29923- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Molecular iodine inhibits the expression of stemness markers on cancer stem-like cells of established cell lines derived from cervical cancer.
Bigoni-Ordóñez GD, Ortiz-Sánchez E, Rosendo-Chalma P, Valencia-González HA, Aceves C, García-Carrancá A
BMC cancer 2018 Sep 26;18(1):928
BMC cancer 2018 Sep 26;18(1):928
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of KLF4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Goat Anti-Human KLF4 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3640A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
