Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 25-0116-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD11c Monoclonal Antibody (3.9), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3.9
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Bovine Lactoferrin Enhances TLR7-Mediated Responses in Plasmacytoid Dendritic Cells in Elderly Women: Results From a Nutritional Intervention Study With Bovine Lactoferrin, GOS and Vitamin D.
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
The Correlation of CD206, CD209, and Disease Severity in Behçet's Disease with Arthritis.
Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells.
dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.
Pharmacological targeting of miR-155 via the NEDD8-activating enzyme inhibitor MLN4924 (Pevonedistat) in FLT3-ITD acute myeloid leukemia.
STAT3 signaling contributes to the high effector activities of interleukin-15-derived dendritic cells.
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
Comprehensive immunophenotyping of cerebrospinal fluid cells in patients with neuroimmunological diseases.
Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα.
Progression from high insulin resistance to type 2 diabetes does not entail additional visceral adipose tissue inflammation.
Peripheral blood CD4 T-cell and plasmacytoid dendritic cell (pDC) reactivity to herpes simplex virus 2 and pDC number do not correlate with the clinical or virologic severity of recurrent genital herpes.
Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.
Antigen-presenting cells represent targets for R5 HIV-1 infection in the first trimester pregnancy uterine mucosa.
van Splunter M, Perdijk O, Fick-Brinkhof H, Feitsma AL, Floris-Vollenbroek EG, Meijer B, Brugman S, Savelkoul HFJ, van Hoffen E, van Neerven RJJ
Frontiers in immunology 2018;9:2677
Frontiers in immunology 2018;9:2677
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
Xiu W, Ma J, Lei T, Zhang M
Oncology letters 2018 Sep;16(3):2851-2856
Oncology letters 2018 Sep;16(3):2851-2856
The Correlation of CD206, CD209, and Disease Severity in Behçet's Disease with Arthritis.
Choi B, Suh CH, Kim HA, Sayeed HM, Sohn S
Mediators of inflammation 2017;2017:7539529
Mediators of inflammation 2017;2017:7539529
Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells.
Sontag S, Förster M, Qin J, Wanek P, Mitzka S, Schüler HM, Koschmieder S, Rose-John S, Seré K, Zenke M
Stem cells (Dayton, Ohio) 2017 Apr;35(4):898-908
Stem cells (Dayton, Ohio) 2017 Apr;35(4):898-908
dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.
Lim S, Kim WJ, Kim YH, Lee S, Koo JH, Lee JA, Yoon H, Kim DH, Park HJ, Kim HM, Lee HG, Yun Kim J, Lee JU, Hun Shin J, Kyun Kim L, Doh J, Kim H, Lee SK, Bothwell ALM, Suh M, Choi JM
Nature communications 2015 Sep 15;6:8244
Nature communications 2015 Sep 15;6:8244
Pharmacological targeting of miR-155 via the NEDD8-activating enzyme inhibitor MLN4924 (Pevonedistat) in FLT3-ITD acute myeloid leukemia.
Khalife J, Radomska HS, Santhanam R, Huang X, Neviani P, Saultz J, Wang H, Wu YZ, Alachkar H, Anghelina M, Dorrance A, Curfman J, Bloomfield CD, Medeiros BC, Perrotti D, Lee LJ, Lee RJ, Caligiuri MA, Pichiorri F, Croce CM, Garzon R, Guzman ML, Mendler JH, Marcucci G
Leukemia 2015 Oct;29(10):1981-92
Leukemia 2015 Oct;29(10):1981-92
STAT3 signaling contributes to the high effector activities of interleukin-15-derived dendritic cells.
Okada S, Han S, Patel ES, Yang LJ, Chang LJ
Immunology and cell biology 2015 May-Jun;93(5):461-71
Immunology and cell biology 2015 May-Jun;93(5):461-71
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
Roberts RA, Eitas TK, Byrne JD, Johnson BM, Short PJ, McKinnon KP, Reisdorf S, Luft JC, DeSimone JM, Ting JP
Biomaterials 2015 Dec;72:1-10
Biomaterials 2015 Dec;72:1-10
Comprehensive immunophenotyping of cerebrospinal fluid cells in patients with neuroimmunological diseases.
Han S, Lin YC, Wu T, Salgado AD, Mexhitaj I, Wuest SC, Romm E, Ohayon J, Goldbach-Mansky R, Vanderver A, Marques A, Toro C, Williamson P, Cortese I, Bielekova B
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2551-63
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2551-63
Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα.
Chao Y, Kaliaperumal N, Chretien AS, Tang S, Lee B, Poidinger M, Fairhurst AM, Connolly JE
Journal of leukocyte biology 2014 Dec;96(6):1037-46
Journal of leukocyte biology 2014 Dec;96(6):1037-46
Progression from high insulin resistance to type 2 diabetes does not entail additional visceral adipose tissue inflammation.
Barbarroja N, Lopez-Pedrera C, Garrido-Sanchez L, Mayas MD, Oliva-Olivera W, Bernal-Lopez MR, El Bekay R, Tinahones FJ
PloS one 2012;7(10):e48155
PloS one 2012;7(10):e48155
Peripheral blood CD4 T-cell and plasmacytoid dendritic cell (pDC) reactivity to herpes simplex virus 2 and pDC number do not correlate with the clinical or virologic severity of recurrent genital herpes.
Moss NJ, Magaret A, Laing KJ, Kask AS, Wang M, Mark KE, Schiffer JT, Wald A, Koelle DM
Journal of virology 2012 Sep;86(18):9952-63
Journal of virology 2012 Sep;86(18):9952-63
Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.
Radomska HS, Alberich-Jordà M, Will B, Gonzalez D, Delwel R, Tenen DG
The Journal of clinical investigation 2012 Aug;122(8):2955-66
The Journal of clinical investigation 2012 Aug;122(8):2955-66
Antigen-presenting cells represent targets for R5 HIV-1 infection in the first trimester pregnancy uterine mucosa.
Marlin R, Nugeyre MT, de Truchis C, Berkane N, Gervaise A, Barré-Sinoussi F, Menu E
PloS one 2009 Jun 22;4(6):e5971
PloS one 2009 Jun 22;4(6):e5971
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD11c PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis.