710309

antibody from Invitrogen Antibodies

Targeting: MMP3 STMY, STMY1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

710309

Provider

Invitrogen Antibodies

Product name

MMP3 Recombinant Polyclonal Antibody (45HCLC)

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Antibody clone number

45HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP3 was performed by loading 20 µg of HeLa (lane1), Hep G2 (lane2), MDA-MB-231 (lane3), Jurkat (lane4) and Raji (lane5) cell lysates using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. MMP3 was detected at ~28 kDa using MMP3 Recombinant Rabbit Polyclonal Antibody (Product # 710309) at 1-2 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP-3 in whole cell extracts from Jurkat (lane 1) and MDAMB 231 cells (lane 2) using a MMP-3 Recombinant Rabbit Polyclonal Antibody (Product # 710309) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~28kDa.