PA5-52301

antibody from Invitrogen Antibodies

Targeting: CPA3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52301

Provider

Invitrogen Antibodies

Product name

Carboxypeptidase A3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RFDREKVFRV KPQDEKQADI IKDLAKTNEL DFWYPGATHH VAANMMVDFR VSEKESQAIQ SALDQNKMHY EILIHDLQEE IEKQFDVKED IPGRHSYAKY NNWEKIVAWT EKMMDKYPEM VSRIKIGSTV EDNPLYVLKI GEKNER

Concentration

0.3 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Carboxypeptidase A3 in control (vector only transfected HEK293T lysate) and CPA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Carboxypeptidase A3 in human small intestine and skeletal muscle tissues using Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301). Corresponding CPA3 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Carboxypeptidase A3 in human cervix, uterine, skeletal muscle, skin and small intestine using Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301) (A) shows similar protein distribution across tissues to an independent Carboxypeptidase A3 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Carboxypeptidase A3 in human small intestine using Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301) shows moderate to strong cytoplasmic positivity in mast cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Carboxypeptidase A3 in human skeletal muscle using Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301) shows very weak positivity in myocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Carboxypeptidase A3 in human cervix, uterine using Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301) shows moderate to strong cytoplasmic positivity in mast cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Carboxypeptidase A3 in human skin using Carboxypeptidase A3 Polyclonal Antibody (Product # PA5-52301) shows moderate to strong cytoplasmic positivity in mast cells.