HPA025041

antibody from Atlas Antibodies

Targeting: EIF4G3 eIF4GII

Antibody data

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Validation data

Product number: HPA025041 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA025041, RRID:AB_1848104
Product name: Anti-EIF4G3
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: VARSTIAAPTSSALSSQPIFTTAIDDRCELSSPRE DTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVA PNDIPLV
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

EIF4G3 protein structure - HPA025041 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and skeletal muscle tissues using Anti-EIF4G3 antibody. Corresponding EIF4G3 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-EIF4G3 antibody HPA025041 (A) shows similar protein distribution across tissues to independent antibody HPA025031 (B).
Antibody #2 product nr: HPA025039, HPA025031
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node using Anti-EIF4G3 antibody HPA025041.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex using Anti-EIF4G3 antibody HPA025041.
Sample type: HUMAN