NBP1-86636

antibody from Novus Biologicals

Targeting: NDRG1 CAP43, DRG1, NDR1, RTP, TDD5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-86636

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-86636, RRID:AB_11015593

Product name

Rabbit Polyclonal NDRG1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, rat NDRG1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

The metastasis suppressor, N-myc downregulated gene 1 (NDRG1), is a prognostic biomarker for human colorectal cancer.

Mao Z, Sun J, Feng B, Ma J, Zang L, Dong F, Zhang D, Zheng M
PloS one 2013;8(7):e68206

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N-myc downstream regulated 1 (NDRG1) is regulated by eukaryotic initiation factor 3a (eIF3a) during cellular stress caused by iron depletion.

Lane DJ, Saletta F, Suryo Rahmanto Y, Kovacevic Z, Richardson DR
PloS one 2013;8(2):e57273

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A deletion in the N-myc downstream regulated gene 1 (NDRG1) gene in Greyhounds with polyneuropathy.

Drögemüller C, Becker D, Kessler B, Kemter E, Tetens J, Jurina K, Jäderlund KH, Flagstad A, Perloski M, Lindblad-Toh K, Matiasek K
PloS one 2010 Jun 22;5(6):e11258

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NDRG4 is required for cell cycle progression and survival in glioblastoma cells.

Schilling SH, Hjelmeland AB, Radiloff DR, Liu IM, Wakeman TP, Fielhauer JR, Foster EH, Lathia JD, Rich JN, Wang XF, Datto MB
The Journal of biological chemistry 2009 Sep 11;284(37):25160-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NDRG1 Antibody [NBP1-86636] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NDRG1 Antibody [NBP1-86636] - Analysis in human cell line U-87 MG.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NDRG1 Antibody [NBP1-86636] - Analysis in human cell lines HEK293 and MCF-7 using Anti-NDRG1 antibody. Corresponding NDRG1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NDRG1 Antibody [NBP1-86636] - Analysis in human cell lines HEK293 and MCF-7. Corresponding NDRG1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human prostate shows strong membranous positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Staining of human colon shows strong membranous positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NDRG1 Antibody [NBP1-86636] - Analysis in human prostate and skeletal muscle tissues. Corresponding NDRG1 RNA-seq data are presented for the same tissues.