Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA1-19291 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EBP50 Monoclonal Antibody (EBP-10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes. A suggested positive control for this product is RAJI human lymphoma cell lysate.
- Antibody clone number
- EBP-10
- Concentration
- 1 mg/mL
Submitted references Immunohistochemical analysis of ezrin-radixin-moesin-binding phosphoprotein 50 in prostatic adenocarcinoma.
Bartholow TL, Becich MJ, Chandran UR, Parwani AV
BMC urology 2011 Jun 14;11:12
BMC urology 2011 Jun 14;11:12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-NHERF1Monoclonal antibody (Product # MA1-19291) followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # MA1-19291) and a 50 kDa band corresponding to EBP50 was observed along with uncharacterized band across cell lines tested. Membrane enriched extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), SK-BR-3 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), HEK-293 (Lane 7)and PC-12 (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Photomicrographs of TMA cores . Photomicrographs of TMA cores (20x). A) NDP, B) BPH, C) NAC, D) HGPIN, E) PCa, and F) Mets. Note the predominantly membranous pattern in BPH, which was noted in cores from 11/15 cases. To a lesser degree, this is also noted in the depicted specimen of HGPIN, although the cytoplasmic staining is also intense, which partially obscures the distinction. In the remainder of depicted cores, the staining is largely cytoplasmic. No membranous staining was noted in any of the metastatic cores.