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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- MA5-26997 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRBP Monoclonal Antibody (OTI7C1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI7C1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of TARBP2 was achieved by transfecting MCF-7 cells with TARBP2 specific siRNAs (Silencer® select Product # s13790). Western blot analysis (Fig. a) was performed using whole cell extracts from the TARBP2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TARBP2 Monoclonal Antibody (Product # MA5-26997, 1:2000 dilution) and chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TARBP2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TARBP2 Monoclonal Antibody (Product # MA5-26997 ) and a 39 kDa band corresponding to TARBP2 was observed along with a probable spliced variant at 21 kDa across cell Lines tested. Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), MCF-7 (Lane 2), LNCaP (Lane 3), and OVCAR-3 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a TARBP2 monoclonal antibody (Product # MA5-26997) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a TARBP2 monoclonal antibody (Product # MA5-26997) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human kidney tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a TARBP2 monoclonal antibody (Product # MA5-26997) at a dilution of 1:150.
