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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP2-47941-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Human Lung SCC stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - PFA-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (blue); Isotype Control (red).
