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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-29978 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal alpha-Internexin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.38 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Internexin Antibody (257CT7.1.2) [NBP2-29978] - Mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Internexin Antibody (257CT7.1.2) [NBP2-29978] - Anti-alpha-Internexin Antibody at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (257CT7.1.2) [NBP2-29978] - INA Antibody (NBP2-29978) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha-Internexin Antibody (257CT7.1.2) [NBP2-29978] - Formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INA Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
