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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- NBP2-50029 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Chicken Polyclonal Calretinin Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation. This antibody does not cross-react with the related calcium binding proteins Calbindin D-28K and Parvalbumin.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calretinin Antibody [NBP2-50029] - Western blot analysis of different tissue lysates using chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to calretinin protein. The same blot was simultaneously probed with mouse mAb to alpha-nternexin, dilution 1:10,000 in red that reveals the alpha-internexin protein with apparent molecular weight of 66kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029] - Analysis of rat hippocampus section stained with chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green, and costained with mouse mAb to parvalbumin, 1:1,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The calretinin and parvalbumin antibodies label different classes of interneurons.
