PA5-85944
antibody from Invitrogen Antibodies
Targeting: RPS6KB1
p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [6]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-85944 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p70 S6 Kinase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p70 S6 Kinase in different lysates using a Polyclonal antibody (Product #PA5-85944) at a dilution of 1:500. Positive control: Lane 1: PC12, Lane 2: NIH/3T3, Lane 3: Jurkat, Lane 4: K562, Lane 5: Hela.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p70 S6 Kinase in Lovo cells using a p70 S6 Kinase Polyclonal antibody (Product # PA5-85944) as seen in green. Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p70 S6 Kinase in NIH/3T3 cells using a p70 S6 Kinase Polyclonal antibody (Product # PA5-85944) as seen in green. Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p70 S6 Kinase in Hela cells using a p70 S6 Kinase Polyclonal antibody (Product # PA5-85944) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p70 S6 Kinase in Lovo cells using a p70 S6 Kinase Polyclonal antibody (Product # PA5-85944) as seen in green. Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p70 S6 Kinase in NIH/3T3 cells using a p70 S6 Kinase Polyclonal antibody (Product # PA5-85944) as seen in green. Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p70 S6 Kinase in Hela cells using a p70 S6 Kinase Polyclonal antibody (Product # PA5-85944) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p70 S6 Kinase of paraffin-embedded rat pancreas tissue using a p70 S6 Kinase Polyclonal antibody (Product #PA5-85944). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p70 S6 Kinase of paraffin-embedded Human colon cancer tissue using a p70 S6 Kinase Polyclonal antibody (Product #PA5-85944). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p70 S6 Kinase of paraffin-embedded Human breast cancer tissue using a p70 S6 Kinase Polyclonal antibody (Product #PA5-85944). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p70 S6 Kinase of paraffin-embedded Mouse pancreas tissue using a p70 S6 Kinase Polyclonal antibody (Product #PA5-85944). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of p70 S6 Kinase in HepG2 cells using a p70 S6 Kinase Polyclonal Antibody (Product # PA5-85944) at a dilution of 1:100, as seen in blue compared with an unlabeled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.