MA5-15044

antibody from Invitrogen Antibodies

Targeting: NQO1 DHQU, DIA4, DTD, NMOR1, QR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15044

Provider

Invitrogen Antibodies

Product name

NQO1 Monoclonal Antibody (F.964.4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

It is not recommended to aliquot this antibody.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

F.964.4

Vial size

100 µL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-NQO1 Monoclonal Antibody (F.964.4) (Product # MA5-15044) and a 31 kDa band corresponding to NQO1 was observed across cell lines tested except SH-SY5Y, MOLT-4 and HEK-293. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), U-87 MG (Lane 4), SH-SY5Y (Lane 5), MOLT-4 (Lane 6) and HEK-293 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NQO1 in HeLa using a NQO1 monoclonal antibody (Product # MA5-15044) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NQO1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NQO1 Monoclonal Antibody (F.964.4) (Product # MA5-15044) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents HEK-293 cells having no expression of NQO1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NQO1 in paraffin-embedded human colon carcinoma using a NQO1 monoclonal antibody (Product # MA5-15044).