17-1339-42

antibody from Invitrogen Antibodies

Targeting: PROM1 AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17-1339-42

Provider

Invitrogen Antibodies

Product name

CD133 (Prominin-1) Monoclonal Antibody (EMK08), APC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The EMK08 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites; the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others.

Antibody clone number

EMK08

Concentration

5 µL/Test

References

Submitted references

Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis.

Ye W, Ni Z, Yicheng S, Pan H, Huang Y, Xiong Y, Liu T
International journal of oncology 2019 Dec;55(6):1296-1312

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Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells.

Guo P, Wang J, Gao W, Liu X, Wu S, Wan B, Xu L, Li Y
Molecular medicine reports 2018 Aug;18(2):1323-1334

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Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.

Chen Q, Khoury M, Chen J
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 22;106(51):21783-8

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A human colon cancer cell capable of initiating tumour growth in immunodeficient mice.

O'Brien CA, Pollett A, Gallinger S, Dick JE
Nature 2007 Jan 4;445(7123):106-10

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Identification of human brain tumour initiating cells.

Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB
Nature 2004 Nov 18;432(7015):396-401

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AC133, a novel marker for human hematopoietic stem and progenitor cells.

Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW
Blood 1997 Dec 15;90(12):5002-12

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Anti-Human CD45 FITC (Product # 11-0459-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human CD133 APC (right). Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Identification of colon CSCs. (A) Flow cytometry analysis and quantification of CD133 expression in spheroid cells and corresponding parental cells. Cells in Q2-2 correspond to CD133-positive cells. Results are representative images of three independent experiments. **P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Proportion of CD44 + /CD133 + cancer stem cells in ovarian cancer tissues of each patient by flow cytometry.